Prevalence of Pseudomonas aeruginosa isolates with molecular identification of some antibiotic resistant genes in Hospitals of Duhok City/ Iraq
Keywords:
Pseudomonas aeruginosa, inpatients, molecular PCR, antibiotic susceptibilityAbstract
Pseudomonas aeruginosa is the major cause of hospital acquired infections in immunocompetent and immunosuppressed individual such as, burn and surgical sites, UTIs, pneumonia, otitis externa, and soft tissues. Nowadays, the emergence of P. aeruginosa isolates that resist metallo-beta-lactamase antibiotics become a serious public health issue globally. The aims of the present investigation are to estimate the prevalence of P. aeruginosa isolates in inpatients, to determine their sensitivity and resistance to the prescribed antibiotics in this area and to identify some of the antibiotic resistant genes. The study was conducted from April 2021 to March 2022, and included 360 samples taken from inpatients admitted to three teaching hospitals (Azadi, Burin and Heevi) and the Vajeen private hospital in Duhok city.
The overall prevalence of Pseudomonas aeruginosa was 30% (108/360). The highest rate in patient samples was 48.78% from sputum, followed by nearly equal rates of 38.78% and 38.3% from both of ears and burns, respectively and the lowest rate 19.34% from urine with highly significant (P<0.001) differences between these sources. The 16 S rDNA gene was amplified by PCR in all isolates, each of them produced a distinct band of 956 bps for this gene. The produced segments of 16S r DNA were sequenced, 4 sequences were deposited in GenBank under accession numbers: OP221013, OP221014, OP221015, OP221016 from sputum, ear, burn and wounds, respectively. In this study also, two antibiotic resistant genes were detected included blaOXA-10 and blaBEL1. The gene blaOXA-10 was present in100% of the isolates, while the genes blaBEL1 was detected in 95.83%. The sequence of blaOXA-10 gene was deposited in GenBank under accession number: OP251042. All isolates carried these genes were 100% resistant to β-lactam antibiotics in addition to Erythromycin and Trimethoprim/Sulfamethoxazole, furthermore about 34% of these genes were detected among ages >50 years.
