Prevalence of Pseudomonas aeruginosa isolates with molecular identification of some antibiotic resistant genes in Hospitals of Duhok City/ Iraq

Authors

  • Siham Abdullah Mohammed Said Department of Pharmacy, Duhok Technical Institute, Polytechnic University, Duhok, Iraq
  • Haval Mohammed Khalid Department of Biology, Faculty of Science, Zakho University, Zakho, Iraq;
  • Wijdan Mohammed Salih Mero Department of Biology, Faculty of Science, Zakho University, Zakho, Iraq; College of Science, Nawroz University, Duhok, Iraq;

DOI:

https://doi.org/10.25007/ajnu.v13n2a2039

Abstract

Pseudomonas aeruginosa is the major cause of hospital acquired infections in immunocompetent and immunosuppressed individual such as, burn and surgical sites, UTIs, pneumonia, otitis externa, and soft tissues. Nowadays, the emergence of P. aeruginosa isolates that resist metallo-beta-lactamase antibiotics become a serious public health issue globally. The aims of the present investigation are to estimate the prevalence of P. aeruginosa isolates in inpatients, to determine their sensitivity and resistance to the prescribed antibiotics in this area and to identify some of the antibiotic resistant genes. The study was conducted from April 2021 to March 2022, and included 360 samples taken from inpatients admitted to three teaching hospitals (Azadi, Burin and Heevi) and the Vajeen private hospital in Duhok city.

The overall prevalence of Pseudomonas aeruginosa was 30% (108/360). The highest rate in patient samples was 48.78% from sputum, followed by nearly equal rates of 38.78% and 38.3% from both of ears and burns, respectively and the lowest rate 19.34% from urine with highly significant (P<0.001) differences between these sources. The 16 S rDNA gene was amplified by PCR in all isolates, each of them produced a distinct band of 956 bps for this gene. The produced segments of 16S r DNA were sequenced, 4 sequences were deposited in GenBank under accession numbers: OP221013, OP221014, OP221015, OP221016 from sputum, ear, burn and wounds, respectively. In this study also, two antibiotic resistant genes were detected included blaOXA-10 and blaBEL1. The gene blaOXA-10 was present in100% of the isolates, while the genes blaBEL1 was detected in 95.83%. The sequence of blaOXA-10 gene was deposited in GenBank under accession number: OP251042. All isolates carried these genes were 100% resistant to β-lactam antibiotics in addition to Erythromycin and Trimethoprim/Sulfamethoxazole, furthermore about 34% of these genes were detected among ages >50 years.

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References

Al-Derzi NA. (2012). Pattern of Resistance to Pseudomonas infection in the North of Iraq: Emphasis on the Potential Role of a Combination Antibiogram. Iraqi Journal of Community Medicine ,25(2): 130-135.

Al-Saffar M F, Jarallah EM. (2019). Isolation and characterization of Pseudomonas aeruginosa from Babylon Province. Biochemical and Cellular Archives,19(1): 203-209.

Bialvaei A Z, Samadi Kafil H. (2015). Colistin, mechanisms and prevalence of resistance. Current Medical Research and Opinion ,31(4): 707- 721.

Ciofu O, Tolker-Nielsen T. (2019). Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents- How P. aeruginosa Can Escape Antibiotics. Frontier Microbiology, 10:913.

CLSI (2021). Performance standards for Antimicrobial Susceptibility Testing. 31st ed. CLSI supplement M100. Clinical and Laboratory Standards Institute. Journal of Clinical Microbiology, 59(12): P16

del-Barrio-Tofiño E, Causapé CL, Oliver A. (2020). Pseudomonas aeruginosa epidemic high-risk clones and their association with horizontally-acquired β-lactamases: 2020 update. International Journal of Antimicrobial Agents, 56(6): 106-196.

Diggle SP, Whiteley M. (2020). Microbe Profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat. Microbiology (Reading) ,166 (1): 30-33.

Emami A, Bazargani A, Mohammadi AA, Zardosht M, Seyed Jafari SM. (2015). Detection of blaPER-1 & blaOxa10 among imipenem resistant isolates of Pseudomonas aeruginosa isolated from burn patients hospitalized in Shiraz Burn Hospital. Iran Journal Microbiology, 7(1):7–11.

Farshadzadeh, Z., Khosravi, A. D., Alavi, S. M., Parhizgari, N., & Hoveizavi, H. (2014). Spread of extended-spectrum β-lactamase genes of bla OXA-10, bla PER-1 and bla CTX-M in Pseudomonas aeruginosa strains isolated from burn patients. Burns, 40(8), 1575-1580.

Finberg RW, Guharoy R. (2021). Monobactam: Aztreonam, in Clinical use of Anti-infective Agents, 2nd edition, Springer Nature, Switzerland.

Folic MM, Djordjevic Z, Folic N, Radojevic MZ, Jankovic SM. (2021) Epidemiology and risk factors for healthcare-associated infections caused by Pseudomonas aeruginosa. Journal of Chemotherapy, 33(5):294-301.

Friyah SH., Rasheed MN. (2018). Molecular Study of Efflux MexX Gene in Pseudomonas aeruginosa Isolated from Iraqi Patients. Iraqi Journal of Biotechnology, 17 (3): 92-99.

Ganjo AR: Antimicrobial susceptibility of extensively drug-resistant (XDR) and multidrug-resistant (MDR) Pseudomonas aeruginosa isolated from patients in Erbil city. The Second International Conference College of Medicine. 2017, HMU, 22nd - 24th, Divan Hotel - Erbil - Kurdistan, Iraq 238.

Hasan SA, Najati AM, Abass, KS. (2019). Isolation and identification of multi-drug resistant “pseudomonas aeruginosa” from burn wound infection in Kirkuk City, Iraq. Eurasian Journal of Biosciences, 13(2): 1045-1050.

Khoshnood S, Khosravi AD, Jomehzadeh N, Montazeri EA, Motahar M, Shahi F, Saki M, Seyed-Mohammadi S. (2019). Distribution of extended-spectrum β-lactamase genes in antibiotic resistant strains of Pseudomonas aeruginosa obtained from burn patients in Ahvaz, Iran. Journal of Acute Diseases, 8(2): 53-57.

Laudy AE, Róg P, Smolińska-Król K, Ćmiel M, Słoczyńska A, Patzer J, Dzierżanowska D, Wolinowska R, Starościak B, Tyski S. (2017). Prevalence of ESBL-producing Pseudomonas aeruginosa isolates in Warsaw, Poland, detected by various phenotypic and genotypic methods. PLoS One,12(6): e0180121.

Lister PD, Wolter DJ, Hanson ND. (2009). Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clinical Microbiology Reviews, 22(4) :582-610.

Maniatis T, Fritsch EF, Sambrook J. (1982). Molecular cloning: A Laboratory Manual Gold Spring Harber Laboratory, New York.

Manjana A, Banjana A, Dipjitdey A Tudu J N. (2016). Selection of Storage Methods for Maintenance of Different Stock Cultures. International Journal of Current Microbiology and Applied Sciences,5(10);1097-1104.

Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. (1999). Aminoglycosides: activity and resistance. Antimicrobial Agents Chemotherapy, 43(4):727–737.

Oliver A, Mulet X, López-Causapé C, Juan C. (2015). The increasing threat of Pseudomonas aeruginosa high-risk clones. Drug Resistance Updates,22:41-59.

Othman N, Babakir-Mina M, Noori CK, Rashid PY. (2014). Pseudomonas aeruginosa infection in burn patients in Sulaymaniyah, Iraq: risk factors and antibiotic resistance rates. Journal Infection in Developing Countries, 8(11): 1498-1502.

Oumeri MMQ, Yassin NA. (2021). Molecular characterization of some carbapenem-resistance genes among Pseudomonas aeruginosa isolated from wound and burn infection in Duhok city, Iraq. Journal of Duhok University ,24(1):136–144.

Pachori P, Gothalwal R, Gandhi P. (2019). Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review. Genes and Diseases, 6(2):109-119.

Pang Z, Raudonis R, Glick B R, Lin T J, Cheng Z. (2019). Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnology advances, 37(1): 177– 192.

Polse RF, Khalid HM, Mero WMS. (2023). Distribution of blaOXA-10, blaPER-1, and blaSHV genes in ESBL-producing Pseudomonas aeruginosa strains isolated from burn patients. Sci Rep 13, 18402.

Rocca, D. M., Aiassa, V., Zoppi, A., Silvero Compagnucci, J., and Becerra, M. C. (2020). Nanostructured gold coating for prevention of biofilm development in medical devices. Journal of Endourology, 34(3), 345-351.

Rosa JMO, Patrice N Laurent P. (2019). ESBLs and resistance to ceftazidime/avibactam and ceftolozane/tazobactam combinations in Escherichia coli and Pseudomonas aeruginosa. Journal of Antimicrobial Chemotherapy, 74(7) :1934-1939.

Saderi H, Lotfalipour H, Owlia P, Salimi H. (2019). Detection of Metallo-β-Lactamase Producing Pseudomonas aeruginosa Isolated from Burn Patients in Tehran, Iran. Laboratory Medicine, 41:609–612.

Senbayrak S, Kolayli F, Inan A, Albayrak GT, Aksaray S, Vahaboglu H. (2015). Clinical significance of OXA-10-type ESBL producing MDR Pseudomonas aeruginosa. Disease and Molecular Medicine, 3(1):1-4.

Shields, P., and Cathcart, L. (2010). Oxidase test protocol. American Society for Microbiology. Link: https://bit. ly/3tpubGp.

Spilker T, Coenye T, Vandamme P, LiPuma JJ. (2004). PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. Journal of clinical microbiology, 42(5): 2074–2079.

Tam VH, Schilling AN, Melnick DA, Coyle E A. (2005). Comparison of beta-lactams in counter-selecting resistance of Pseudomonas aeruginosa. Diagnostic Microbiology and Infectious Diseases, 52(2):145-151.

Thalhammer F, Hörl W H. (2000). Pharmacokinetics of meropenem in patients with renal failure and patients receiving renal replacement therapy. Clinical Pharmacokinetics Journal,39(4):271-279.

Torrecillas M, Fuster B, Belda M, Del –Remedio- Guna M, Tormo N, Gimeno C. (2020). Evaluation of a mass spectrometry and Vitec 2 combined protocol for rapid identification and susceptibility testing of enterobacterales directly from positive blood cultures. Infirm Infect Microbiology Clinic (English education),38(8), 375–378.

Tsao LH, Hsin CY, Liu HY, Chuang HC, Chen LY, Lee YJ. (2018). Risk factors for healthcare-associated infection caused by carbapenem-resistant Pseudomonas aeruginosa. Journal of Microbiology Immunology Infection,51(3): 359-366.

WHO (2002). Humans: Reducing risks promoting healthy life. World Health Organization report.

Yassin NA, Khalid HM, Hassan AO. (2014). Prevalence of metallo-β-lactamase producing Pseudomonas aeruginosa in wound infections in Duhok City, Iraq. International Journal of Research in Medical Sciences, 2(4):1576-1579.

Yoshimura F, Nikaido H. (1982). Permeability of Pseudomonas aeruginosa outer membrane to hydrophilic solutes. Journal of bacteriology, 152(2): 636-642.

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Published

2024-06-30

How to Cite

Abdullah Mohammed Said , S., Mohammed Khalid, H. ., & Mohammed Salih Mero, W. (2024). Prevalence of Pseudomonas aeruginosa isolates with molecular identification of some antibiotic resistant genes in Hospitals of Duhok City/ Iraq. Academic Journal of Nawroz University, 13(2), 1333–1342. https://doi.org/10.25007/ajnu.v13n2a2039

Issue

Vol. 13 No. 2 (2024)

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